Date
Wednesday, July 29, 2026
Time
2:50 PM - 3:20 PM (MDT)
Name
Nickase Cas9 Unsilences Paternal Ube3a in a Mouse Model of Angelman Syndrome Without Causing AAV Vector Integration
Description
Mark Zylka PhD Submitted:
We found that a nickase Neisseria meningitidis Cas9 variant (nNmCas9-D15A) disrupted Ube3a-ATS transcription when targeted to the non-template strand and unsilenced patUbe3a in cultured neurons without generating DSBs or causing AAV integration.
Location Name
Colorado B
Speakers
Moderator(s)
Becky Burdine, Dylan Ritter
Session Type
Presentation